Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines.
作者： Bao-Hui Liu , Ling-Min Shao , Qian-Xue Chen , Ting-Feng Wu , Ji-Qiang Li
DOI:
关键词: Medicine 、 Cell 、 Viability assay 、 Puerarin 、 Apoptosis 、 Cell cycle 、 Cell culture 、 Cell growth 、 Flow cytometry 、 Immunology 、 Molecular biology
摘要: Puerarin has been widely used in clinical treatment and experiment research is considered to exert an anticancer effect recently. The present study investigated the activity of puerarin U251 U87 human glioblastoma cells. cells were treated with at various concentrations for different times. Cell viability cell proliferation detected by counting kit-8 (CCK-8) assay 5-ethynyl-2’-deoxyuridine (EdU) staining respectively. cycle apoptosis measured separately PI Annexin V-FITC/PI double method flow cytometry. DNA damage caused exposure was evaluated γ-H2AX foci detection, expressions p-AKT, caspase-3 apoptosis-related proteins Western blotting after treatment. significantly lower than that control group; rate increased obviously compared group. decreased proportion G1 phase cycling accompanied populations S G2/M phases both lines. At same time, level higher Moreover, suppressed expression p-Akt Bcl-2 promoted Bax cleaved These findings indicate exerts antitumor effects
ijcem.com 参考文章(35)
Gerard I. Evan, Karen H. Vousden, Proliferation, cell cycle and apoptosis in cancer Nature. ,vol. 411, pp. 342- 348 ,(2001) , 10.1038/35077213
Qin Wang, Taixiang Wu, Xiao Y Chen, Xin Duan, Jie Zheng, Jieqi Qiao, Likun Zhou, Jiafu Wei, Juan Ni, Puerarin injection for unstable angina pectoris Cochrane Database of Systematic Reviews. ,(2016) , 10.1002/14651858.CD004196.PUB2
Yan-Xi Zhou, Hong Zhang, Cheng Peng, Puerarin: A Review of Pharmacological Effects Phytotherapy Research. ,vol. 28, pp. 961- 975 ,(2014) , 10.1002/PTR.5083
David N. Louis, WHO classification of tumours of the central nervous system International Agency for Research on Cancer. ,(2007)
Jiarui Wu, Xiaomeng Zhang, Bing Zhang, Efficacy and safety of puerarin injection in treatment of diabetic peripheral neuropathy: a systematic review and Meta-analysis of randomized controlled trials Journal of Traditional Chinese Medicine. ,vol. 34, pp. 401- 410 ,(2014) , 10.1016/S0254-6272(15)30039-X
Michitaka Numoto, Akihiro Ito, Tetsuya Toge, Kazuyoshi Yanagihara, Antiproliferative Effects of Isoflavones on Human Cancer Cell Lines Established from the Gastrointestinal Tract Cancer Research. ,vol. 53, pp. 5815- 5821 ,(1993)
H. Dorota Halicka, Xuan Huang, Frank Traganos, Malcolm A. King, Wei Dai, Zbigniew Darzynkiewicz, Histone H2AX phosphorylation after cell irradiation with UV-B: relationship to cell cycle phase and induction of apoptosis. Cell Cycle. ,vol. 4, pp. 339- 345 ,(2005) , 10.4161/CC.4.2.1486
Maozhou Gan, Xiaodong Yin, Puerarin induced in mantle cell lymphoma apoptosis and its possible mechanisms involving multi-signaling pathway. Cell Biochemistry and Biophysics. ,vol. 71, pp. 367- 373 ,(2015) , 10.1007/S12013-014-0207-Y
Sang-Bum Kim, In-Soo Yoon, Kyu-Sang Kim, Sung-Jun Cho, Yeong Kim, Hyun-Jong Cho, Suk-Jae Chung, Saeho Chong, Dae-Duk Kim, In vitro and in vivo evaluation of the effect of puerarin on hepatic cytochrome p450-mediated drug metabolism. Planta Medica. ,vol. 80, pp. 561- 567 ,(2014) , 10.1055/S-0034-1368350
Wing Ming Keung, Bert L. Vallee, Kudzu root: An ancient chinese source of modern antidipsotropic agents Phytochemistry. ,vol. 47, pp. 499- 506 ,(1998) , 10.1016/S0031-9422(97)00723-1