Contact Effect of a Methylobacterium sp. Extract on Biofilm of a Mycobacterium chimaera Strain Isolated from a 3T Heater-Cooler System
作者： Inés Pradal , Jaime Esteban , Arancha Mediero , Marta García-Coca , John Jairo Aguilera-Correa
DOI: 10.3390/ANTIBIOTICS9080474
关键词: Xanthine oxidase 、 Viability assay 、 Microbiology 、 Methylobacterium 、 Mycobacterium 、 Extracorporeal membrane oxygenator 、 Dehydration 、 Methylobacterium sp. 、 Chemistry 、 Biofilm
摘要: Mycobacterium chimaera is an opportunistic slowly growing non-tuberculous mycobacteriumof increasing importance due to the outbreak of cases associated with contaminated 3T heater-cooler device (HCD) extracorporeal membrane oxygenator (ECMO). The aim this study was evaluate effect pre-treating a surface Methylobacterium sp. CECT 7180 extract inhibit M. ECMO biofilm as well treatment after different dehydration times. Surface adherence, formation and were evaluated by estimating colony-forming units (CFU) per square centimeter characterizing amount covered area, thickness, cell viability, presence intrinsic autofluorescence at times using confocal laser scanning microscopy image analysis. We found that exposing inhibited development. This could be result proteins, such DNaK, trigger factor, xanthine oxidase. In conclusion, Methylobacteriumsp. inhibits Furthermore, used pre-treatment prior disinfection protocols for equipment mycobacteria least 96 h.
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sci-hub.st 参考文章(57)
John A. Odell, Margaret M. Johnson, Nontuberculous mycobacterial pulmonary infections Journal of Thoracic Disease. ,vol. 6, pp. 210- 220 ,(2014) , 10.3978/J.ISSN.2072-1439.2013.12.24
Anne Mai-Prochnow, Jeremy S. Webb, Belinda C. Ferrari, Staffan Kjelleberg, Ecological Advantages of Autolysis during the Development and Dispersal of Pseudoalteromonas tunicata Biofilms Applied and Environmental Microbiology. ,vol. 72, pp. 5414- 5420 ,(2006) , 10.1128/AEM.00546-06
C Balachandran, V Duraipandiyan, S Ignacimuthu, Cytotoxic (A549) and antimicrobial effects of Methylobacterium sp. isolate (ERI-135) from Nilgiris forest soil, India Asian pacific Journal of Tropical Biomedicine. ,vol. 2, pp. 712- 716 ,(2012) , 10.1016/S2221-1691(12)60215-9
Robert S. Pitcher, Andrew J. Green, Anna Brzostek, Malgorzata Korycka-Machala, Jaroslaw Dziadek, Aidan J. Doherty, NHEJ protects mycobacteria in stationary phase against the harmful effects of desiccation. DNA Repair. ,vol. 6, pp. 1271- 1276 ,(2007) , 10.1016/J.DNAREP.2007.02.009
Hugo Sax, Guido Bloemberg, Barbara Hasse, Rami Sommerstein, Philipp Kohler, Yvonne Achermann, Matthias Rössle, Volkmar Falk, Stefan P Kuster, Erik C Böttger, Rainer Weber, None, Prolonged Outbreak of Mycobacterium chimaera Infection After Open-Chest Heart Surgery Clinical Infectious Diseases. ,vol. 61, pp. 67- 75 ,(2015) , 10.1093/CID/CIV198
Atsuko Tsujii, Takeshi Nishino, Mechanism of transition from xanthine dehydrogenase to xanthine oxidase: effect of guanidine-HCL or urea on the activity. Nucleosides, Nucleotides & Nucleic Acids. ,vol. 27, pp. 881- 887 ,(2008) , 10.1080/15257770802146569
María-Carmen Muñoz-Egea, María García-Pedrazuela, Ignacio Mahillo, María Jesús García, Jaime Esteban, Autofluorescence as a Tool for Structural Analysis of Biofilms Formed by Nonpigmented Rapidly Growing Mycobacteria Applied and Environmental Microbiology. ,vol. 79, pp. 1065- 1067 ,(2013) , 10.1128/AEM.03149-12
H. TOMIOKA, K. SATO, C. SANO, T. AKAKI, T. SHIMIZU, H. KAJITANI, H. SAITO, Effector molecules of the host defence mechanism against Mycobacterium avium complex: the evidence showing that reactive oxygen intermediates, reactive nitrogen intermediates, and free fatty acids each alone are not decisive in expression of macrophage antimicrobial activity against the parasites Clinical and Experimental Immunology. ,vol. 109, pp. 248- 254 ,(1997) , 10.1046/J.1365-2249.1997.4511349.X
Jeng Yeong Chow, Long Wu, Wen Shan Yew, Directed evolution of a quorum-quenching lactonase from Mycobacterium avium subsp. paratuberculosis K-10 in the amidohydrolase superfamily. Biochemistry. ,vol. 48, pp. 4344- 4353 ,(2009) , 10.1021/BI9004045
Andrej Shevchenko, Matthias Wilm, Ole Vorm, Matthias Mann, None, Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels Analytical Chemistry. ,vol. 68, pp. 850- 858 ,(1996) , 10.1021/AC950914H