Chapter 19 Preparation of Microcells

摘要: Publisher Summary This chapter outlines the techniques for producing subcellular fragments called microcells. These consist of a sub-diploid micronucleus surrounded by rim cytoplasm and plasma membrane. The describes properties microcells discusses potential value experiments where they are fused with normal or mutant cells. method preparation depends on two distinct processes: (1) production populations micronucleate cells (2) application enucleation to these An alternative is subject synchronized in mitosis cold shock. induces abnormal cleavage when returned temperatures. Microcells also form spontaneously small numbers cell cultures treated colchicine. microcell hybrids field chromosome mapping. provide direct source heterogeneous clones hybrid reduced complement from donor without inevitable uncertain delays at present encountered some systems.