MiR-26a functions oppositely in osteogenic differentiation of BMSCs and ADSCs depending on distinct activation and roles of Wnt and BMP signaling pathway

摘要: MicroRNAs (miRNAs) emerge as important regulators of stem cell lineage commitment and bone development. MiRNA-26a (miR-26a) is one the miRNAs regulating osteogenic differentiation both marrow-derived mesenchymal cells (BMSCs) adipose tissue-derived (ADSCs). However, miR-26a functions oppositely in BMSCs ADSCs, suggesting distinct post-transcriptional regulation tissue-specific MSC differentiation. molecular basis largely unknown. Here, we report that function depended on intrinsic signaling network MSCs. Using bioinformatics functional assay, confirmed potentially targeted GSK3β Smad1 to regulate Wnt BMP pathway. Overall comparative analysis revealed was enhanced more potently played a role than BMSCs, whereas pathway essential for promoting ADSCs. The activation pattern pathways determined majorly activate it inhibited suppress interfering with Taken together, our study demonstrated ADSCs applied different facilitate their differentiation, which inverse miR-26a. transcriptional suggested differences between MSCs complexity research MSC-based therapy.