Method for simultaneous luminescence sensing of two species using optical probes of different decay time, and its application to an enzymatic reaction at varying temperature

摘要: Chemical sensing, imaging and microscopy based on the use of fluorescent probes has so far been limited almost exclusively to detection a single parameter at time. We present scheme that can overcome this limitation by enabling optical sensing two simultaneously even identical excitation emission wavelengths provided (a) their decay times are different enough enable time windows be recorded, (b) shorter-lived probe decays below detectable limit while other still measured. refer new as dual lifetime determination (DLD) method show it widely varied appropriate choice experimental settings. DLD is demonstrated work oxygen temperature independently from each making probes, one for (a platinum porphyrin dissolved in polystyrene), [a europium complex poly(vinyl methylketone)]. was applied monitor consumption glucose oxidase-catalyzed oxidation varying temperatures. The expected have further applications cellular assays biophysical imaging.