SKY and FISH analysis of radiation-induced chromosome aberrations: a comparison of whole and partial genome analysis.

摘要: For a retrospective dose estimation of human exposure to ionising radiation, partial genome analysis is routinely used quantify radiation-induced chromosome aberrations. this purpose, fluorescence in situ hybridisation (FISH) with whole painting probes for selected chromosomes usually applied covering about 20% the genome. Since genome-wide screening techniques like spectral karyotyping (SKY) and multiplex FISH (mFISH) have been developed detection aberrations within has now become feasible. To determine correspondence between aberrations, they were measured comprehensively study using vitro irradiated blood samples from three donors. We able demonstrate that comparable results can be detected both approaches. However, complex might misinterpreted by analysis. therefore conclude SKY useful especially high range correct aberration data exchange