A novel in vitro ELISA for estimation of glycoprotein content in human rabies vaccines.
作者： Shukra M. Aavula , Gontu Abhinay , Sridevi V. Nimmagadda , Kapil Maithal
DOI: 10.1080/15321819.2017.1295056
关键词: Rabies 、 Glycoprotein 、 Monoclonal antibody 、 Potency 、 Biology 、 Rabies vaccine 、 In vivo 、 Recombinant DNA 、 Virology 、 In vitro
摘要: In vitro methods for quantification of immunodominant glycoprotein in the rabies vaccine formulations serve as good alternative to cumbersome and variable mice potency assay a batch release test vaccine. The present study presents development sandwich ELISA with optimal concentrations high affinity recombinant diabody (D06) specific monoclonal antibody (M5B4) against its formulations. estimate correlated linearly (r2 = 0.8) vivo This promises forecast values thereby can simple, yet effective vaccines replacing assay.
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