Biocomputational prediction of small non-coding RNAs in Streptomyces.
作者： Josef Pánek , Jan Bobek , Karel Mikulík , Marek Basler , Jiří Vohradský
关键词: DNA microarray 、 Streptomyces 、 Genetics 、 Genome 、 Biology 、 Streptomyces coelicolor 、 Non-coding RNA 、 Actinomyces 、 RNA 、 Gene
摘要: The first systematic study of small non-coding RNAs (sRNA, ncRNA) in Streptomyces is presented. Except for a few exceptions, the sRNAs, as well sRNAs other genera Actinomyces group, have remained unstudied. This was based on sequence conservation intergenic regions Streptomyces, localization transcription termination factors, and genomic arrangement genes flanking predicted sRNAs. Thirty-two potential were predicted. Of these, expression 20 detected by microarrays RT-PCR. prediction validated structure computational approach. Two found to be terminated factors different from Rho-independent terminators. One sRNA identified computationally with high probability 6S RNA. Out 32 24 structurally dissimilar known largest genus Actinomyces, whose not been studied. group bacterial species unique genomes phenotypes. Therefore, new may identified. structural dissimilarity demonstrated this serve evidence uniqueness
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