Ankyrin repeat domain 28 (ANKRD28), a novel binding partner of DOCK180, promotes cell migration by regulating focal adhesion formation
作者： Mitsuhiro Tachibana , Etsuko Kiyokawa , Shigeo Hara , Shun-ichiro Iemura , Tohru Natsume
DOI: 10.1016/J.YEXCR.2008.12.005
关键词: Ankyrin repeat 、 Cell biology 、 SH3 domain 、 Focal adhesion 、 Crk-Associated Substrate Protein 、 Paxillin 、 Signal transducing adaptor protein 、 Biology 、 Adapter molecule crk 、 Dock180
摘要: DOCK180 is a guanine exchange factor of Rac1 originally identified as protein bound to an SH3 domain the Crk adaptor protein. induces tyrosine phosphorylation p130(Cas), and recruits Crk-p130(Cas) complex focal adhesions. To understand role in cell adhesion migration, we searched for DOCK180-binding proteins with nano-LC/MS/MS system, ANKRD28, that contains twenty-six ankyrin repeats. Knockdown ANKRD28 by RNA interference reduced velocity migration HeLa cells, suggesting this plays physiologic DOCK180-Rac1 signaling pathway. Furthermore, knockdown was found alter distribution such Crk, paxillin, p130(Cas). On other hand, expression induced hyper-phosphorylation impaired detachment membrane during migration. Consequently, cells expressing exhibited multiple long cellular processes. associated SH3-dependent manner competed ELMO, another DOCK180. In striking contrast overexpression ELMO extensive lamellipodial protrusion around entire circumference. These data suggest specifies localization activity
elsevier.com
sci-hub.se 参考文章(38)
Peter W. Reddien, H. Robert Horvitz, CED-2/CrkII and CED-10/Rac control phagocytosis and cell migration in Caenorhabditis elegans. Nature Cell Biology. ,vol. 2, pp. 131- 136 ,(2000) , 10.1038/35004000
Louisa P. Wu, Kathryn V. Anderson, Regulated nuclear import of Rel proteins in the Drosophila immune response Nature. ,vol. 392, pp. 93- 97 ,(1998) , 10.1038/32195
R. Sakai, A. Iwamatsu, N. Hirano, S. Ogawa, T. Tanaka, H. Mano, Y. Yazaki, H. Hirai, A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner. The EMBO Journal. ,vol. 13, pp. 3748- 3756 ,(1994) , 10.1002/J.1460-2075.1994.TB06684.X
Jean-François Côté, Andrea B Motoyama, Jason A Bush, Kristiina Vuori, None, A novel and evolutionarily conserved PtdIns(3,4,5)P3-binding domain is necessary for DOCK180 signalling. Nature Cell Biology. ,vol. 7, pp. 797- 807 ,(2005) , 10.1038/NCB1280
Tohru Natsume, Yoshio Yamauchi, Hiroshi Nakayama, Takashi Shinkawa, Mitsuaki Yanagida, Nobuhiro Takahashi, Toshiaki Isobe, A direct nanoflow liquid chromatography-tandem mass spectrometry system for interaction proteomics. Analytical Chemistry. ,vol. 74, pp. 4725- 4733 ,(2002) , 10.1021/AC020018N
Mitsuko Watabe-Uchida, Keisha A. John, Justyna A. Janas, Sarah E. Newey, Linda Van Aelst, The Rac Activator DOCK7 Regulates Neuronal Polarity through Local Phosphorylation of Stathmin/Op18 Neuron. ,vol. 51, pp. 727- 739 ,(2006) , 10.1016/J.NEURON.2006.07.020
Yasuhiro Sawada, Masako Tamada, Benjamin J. Dubin-Thaler, Oksana Cherniavskaya, Ryuichi Sakai, Sakae Tanaka, Michael P. Sheetz, Force Sensing by Mechanical Extension of the Src Family Kinase Substrate p130Cas Cell. ,vol. 127, pp. 1015- 1026 ,(2006) , 10.1016/J.CELL.2006.09.044
K. M. Nolan, K. Barrett, Y. Lu, K.-Q. Hu, S. Vincent, J. Settleman, Myoblast city, the Drosophila homolog of DOCK180/CED-5, is required in a Rac signaling pathway utilized for multiple developmental processes Genes & Development. ,vol. 12, pp. 3337- 3342 ,(1998) , 10.1101/GAD.12.21.3337
Nahum Meller, Mohammad Irani-Tehrani, William B. Kiosses, Miguel A. Del Pozo, Martin A. Schwartz, Zizimin1, a novel Cdc42 activator, reveals a new GEF domain for Rho proteins. Nature Cell Biology. ,vol. 4, pp. 639- 647 ,(2002) , 10.1038/NCB835
Gwangrog Lee, Khadar Abdi, Yong Jiang, Peter Michaely, Vann Bennett, Piotr E. Marszalek, Nanospring behaviour of ankyrin repeats Nature. ,vol. 440, pp. 246- 249 ,(2006) , 10.1038/NATURE04437