Chromium(VI)-reducing Chlorella spp. isolated from disposal sites of paper-pulp and electroplating industry
作者： Swati N. Yewalkar , Kondiram. N. Dhumal , Jayashree K. Sainis
DOI: 10.1007/S10811-007-9153-Z
关键词: Algae 、 Chromium 、 Nuclear chemistry 、 Cell wall 、 Chlorella 、 Chromate conversion coating 、 Sodium azide 、 Biology 、 Green algae 、 Urea 、 Microbiology 、 Plant science 、 Aquatic science
摘要: We isolated four cultures of chromate resistant, unicellular, non-motile green algae from disposal sites the paper-pulp and electroplating industries. These were maintained in Tris-acetate-glycerophosphate medium containing 30 μM K2Cr2O7. The morphological features as well analysis 500-bp fragment 18S rDNA (NS 12 region) showed that these isolates belong to Chlorella spp. EC50 values for ranging 60 125 μM. Uptake studies with radioactive 51Cr(VI) 10–19% total radioactivity was intracellular, 1–2% bound cell wall. rest activity remained medium, suggesting resistance not related accumulation Cr(VI) cells. Interestingly, when grown presence K2Cr2O7, a decrease concentration observed. Only live cells could deplete supernatant, chromium reduction isolates. stimulated by light acetate glycerophosphate. Treatment 3-(3,4 dichlorophenyl),1,1dimethyl urea (DCMU) did affect reduction. However, if treated sodium azide, severely affected. Though has been documented algae, information on is scant. This paper discusses resistant Chlorella, which may find use effective bioremediation Cr(VI).
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