Two-stage screening of combinatorial peptide libraries. Application to bovine serum albumin ligand selection
作者： Fernando Albericio , María Camila Martínez-Ceron , Osvaldo Cascone , Mariela Mirta Marani , Silvia Andrea Camperi
DOI:
关键词: Bovine serum albumin 、 Texas Red 、 Amino acid 、 Ligand 、 Fluorescence 、 Peptide 、 Combinatorial chemistry 、 Peptide library 、 Target protein 、 Chemistry
摘要: Short peptides are excellent ligands for affinity chromatrography. Divide-couple- recombine method allows obtaining a peptide library with all possible combinations of the amino acids in form "one bead-one peptide". It was designed two-stage screening selection this work. The performed target protein (bovine seroalbumin, BSA) coupled to Texas Red. Fluorescent beads were automatically
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