Potential Regulation of Ste20 Function by the Cln1-Cdc28 and Cln2-Cdc28 Cyclin-dependent Protein Kinases

摘要: The activity of the Saccharomyces cerevisiae pheromone signal transduction pathway is regulated by Cln1/2-Cdc28 cyclin-dependent kinase. High level expression CLN2 can repress activation mating factor or deletion α-subunit heterotrimeric G-protein. We now show that overexpression also FUS1 induction if signaling activated at β-subunit G-protein (STE4) but not when downstream kinases (STE20 and STE11) transcription STE12. This epistatic analysis indicates repression Cln2-Cdc28 kinase takes place a around STE20. In agreement with this, marked reduction in electrophoretic mobility Ste20 protein observed time cell cycle maximal CLN2. change constitutive cells overexpressing absent lackingCLN1 These changes correlate suggest as target for G1cyclins. Two morphogenic pathways which essential, pseudohyphal differentiation haploid-invasive growth, requireCLN1 Together previous observation Cln1 Cln2 are required function cytokinesis, this suggests regulate biological promoting functions, while inhibiting function.