On the Conformation of Pyridoxal Phosphate Imine in Solution and in Aspartate-Aminotransferase Active Site

摘要: The work comprises: 1 Determination by nuclear magnetic resonance method of the conformation imino group pyridoxal-P oxime in solution. It has been found to be nearly coplanar with aromatic ring. 2 Theoretical calculation atom-atom potentials permitted conformations imine and its analogs: 6-methylpyridoxal-P 5′-methylpyridoxal-P. There have seven favourable for imine, some which were prohibited analogs mentioned. 3 Experimental estimation active site aspartate aminotransferase use above sterically hindered 5′-deoxy-5′-carboxy-methylenepyridoxal analog. In result, number possible reduced a pair symmetrical conformations.