作者： Gerstein , Karpikov , Mahajan , Hartman , Snyder
DOI:
关键词: Polyadenylation 、 RNA polymerase binding 、 DNA 、 RNA polyadenylation 、 RNA polymerase II 、 RNA polymerase 、 RNA 、 Biology 、 Polymerase 、 Cell biology
摘要: Genomic analyses have been applied extensively to analyze the process of transcription initiation in mammalian cells, but much less events associated with transcript 3’ end formation and termination. We used a novel approach prepare fragment libraries from polyadenylated RNA several cell types, globally mapped position poly(A) addition site using oligonucleotide arrays tiling one percent human genome. This revealed more ends than had previously annotated. The distribution these relative DNA sites bound by polymerase II distributions those for diand trimethylated lysine 4 36 histone 3, was compared ChIP-chip analysis. found that substantial fraction unannotated are intronic reside antisense embedding gene. Poly(A) annotated messages lie at variable distance averaging approximately two kb upstream binding (termination). Near termination, as well some internal sites, there is an accumulation both unphosphorylated carboxy-terminal domain (CTD) serine 2 phosphorylated large subunit II, suggesting pausing perhaps dephosphorylation prior release. Lysine trimethylation occurs across body many transcribed genes, sometimes alternating stretches which lysine36 dimethylation relatively prominent. methylation often decreases beginning or near polyadenylation, disappearing before disappearance release DNA. Our results suggest termination may involve separable loss histone3 later polymerase. latter Thus, overall our study reveals extensive genome provides insights into occur during formation. INTRODUCTION Identification regions encode transcripts essential complete functional understanding function Studies over last few years can be accounted