Efecto del lipopolisacárido en cultivos de células dendríticas humanas y su inhibición por la polimixina B

摘要: Dendritic cells have been described as effective antigen presenting cells. Human dentritic are highly susceptible to lipopolysaccharide (LPS) tolerance, consisting of a differential deactivation state in which some cellular functions impaired. LPS tolerance can be experimentally induced vitro, the presence strongly affects behavior cultured dendritic Recombinant proteins obtained from bacterial systems or protein extracts ectoparasites containing used stimuli enhance maturation processes these The present study evaluated effect human cell cultures, and activity polymyxin B an inhibitor effect. were peripheral blood monocytes IL-4 GM-CSF, followed by exposure with PGE2/TNFalpha. Surface markers cytokine levels flow cytometry. pre-exposed single doses endotoxin demonstrated reduced capacity mature, CD83 expression, inhibited secretion IL-12, TNFalpha, IL-10 diminished IL-6. Furthermore, at 10 mg/ml inhibits 1 mg/ml. maximum concentration no on morphology was 50 Consequently, determined cultures.