Development of microparticulate systems for intestinal delivery of Lactobacillus acidophilus and Bifidobacterium lactis

摘要: In the present study intestinal delivery systems resistant to gastric juice, loaded with probiotic bacteria Lactobacillus acidophilus LA14 and Bifidobacterium lactis BI07, were produced by polyelectrolyte complexation. First, beads prepared traditional extrusion method nine formulations developed using alginate as main carrier biopolymer, xanthan gum (XG), hydrophilic retardant polymer or cellulose derivative, acetate phthalate (CAP), gastro-resistant polymer. The results showed that incorporation of 0.5% (w/v) XG 1% CAP within 3% solution increased survival in acid conditions from 63% freeze-dried up 76%. Subsequently, these formula was used prepare smaller microcapsules means an atomization device. Despite high viscosity biomass suspension, spraying system spherical non-aggregated able survive harsh condition better than beads: after incubation 91%. performance simulated fluid (SGF) containing pepsin gut medium (GM) bile salts excellent (viability>95%). Furthermore, viability maintained 24h GM. Finally, stability tests performed at 5 degrees C highlighted a bacterial about 82% 70% 6 9 months, respectively.