作者: Rose M. Jones , D. Barrie Johnson
DOI: 10.1016/J.RESMIC.2015.01.003
关键词: Strain (chemistry) 、 Ferrous 、 Heterotroph 、 Acidithrix ferrooxidans 、 Biology 、 Acidophile 、 Botany 、 16S ribosomal RNA 、 Sulfur 、 Actinobacteria 、 Microbiology
摘要: Abstract A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining abandoned copper mine in north Wales. The isolate (PY-F3) demonstrated to be a heterotroph that catalyzed oxidation ferrous iron (but not sulfur or hydrogen) under aerobic conditions, and reduction ferric micro-aerobic anaerobic conditions. PY-F3 formed long entangled filaments cells (>50 μm long) during active growth phases, though these degenerated into smaller fragments single late stationary phase. Although observed grow below pH 2.0 10 °C, harvested biomass found oxidize at relatively fast rates 1.5 5 °C. Phylogenetic analysis, based on comparisons 16S rRNA gene sequences, showed has 91–93% similarity those four classified genera species , therefore is representative genus. binomial Acidithrix ferrooxidans proposed for this new species, with as designated type strain (=DSM 28176 T =JCM 19728 ).