Acidithrix ferrooxidans gen. nov., sp. nov.; a filamentous and obligately heterotrophic, acidophilic member of the Actinobacteria that catalyzes dissimilatory oxido-reduction of iron.

作者: Rose M. Jones , D. Barrie Johnson

DOI: 10.1016/J.RESMIC.2015.01.003

关键词: Strain (chemistry)FerrousHeterotrophAcidithrix ferrooxidansBiologyAcidophileBotany16S ribosomal RNASulfurActinobacteriaMicrobiology

摘要: Abstract A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining abandoned copper mine in north Wales. The isolate (PY-F3) demonstrated to be a heterotroph that catalyzed oxidation ferrous iron (but not sulfur or hydrogen) under aerobic conditions, and reduction ferric micro-aerobic anaerobic conditions. PY-F3 formed long entangled filaments cells (>50 μm long) during active growth phases, though these degenerated into smaller fragments single late stationary phase. Although observed grow below pH 2.0 10 °C, harvested biomass found oxidize at relatively fast rates 1.5 5 °C. Phylogenetic analysis, based on comparisons 16S rRNA gene sequences, showed has 91–93% similarity those four classified genera species , therefore is representative genus. binomial Acidithrix ferrooxidans proposed for this new species, with as designated type strain (=DSM 28176 T =JCM 19728 ).

参考文章(30)
D. Barrie Johnson, Kevin B. Hallberg, Techniques for Detecting and Identifying Acidophilic Mineral-Oxidizing Microorganisms Springer, Berlin, Heidelberg. pp. 237- 261 ,(2007) , 10.1007/978-3-540-34911-2_12
D B Johnson, M A Ghauri, M F Said, Isolation and characterization of an acidophilic, heterotrophic bacterium capable of oxidizing ferrous iron. Applied and Environmental Microbiology. ,vol. 58, pp. 1423- 1428 ,(1992) , 10.1128/AEM.58.5.1423-1428.1992
D.W. Blowes, C.J. Ptacek, J.L. Jambor, C.G. Weisener, D. Paktunc, W.D. Gould, D.B. Johnson, The geochemistry of acid mine drainage Treatise on Geochemistry. ,vol. 11, pp. 131- 190 ,(2014) , 10.1016/B978-0-08-095975-7.00905-0
E. L. J. Watkin, S. E. Keeling, F. A. Perrot, D. W. Shiers, M.-L. Palmer, H. R. Watling, Metals tolerance in moderately thermophilic isolates from a spent copper sulfide heap, closely related to Acidithiobacillus caldus, Acidimicrobium ferrooxidans and Sulfobacillus thermosulfidooxidans Journal of Industrial Microbiology & Biotechnology. ,vol. 36, pp. 461- 465 ,(2009) , 10.1007/S10295-008-0508-5
P.D. Franzmann, C.M. Haddad, R.B. Hawkes, W.J. Robertson, J.J. Plumb, Effects of temperature on the rates of iron and sulfur oxidation by selected bioleaching Bacteria and Archaea: Application of the Ratkowsky equation Minerals Engineering. ,vol. 18, pp. 1304- 1314 ,(2005) , 10.1016/J.MINENG.2005.04.006
A. Schippers, A. Breuker, A. Blazejak, K. Bosecker, D. Kock, T.L. Wright, The biogeochemistry and microbiology of sulfidic mine waste and bioleaching dumps and heaps, and novel Fe(II)-oxidizing bacteria Hydrometallurgy. ,vol. 104, pp. 342- 350 ,(2010) , 10.1016/J.HYDROMET.2010.01.012