Enhancing the mechanical properties and physical stability of biomimetic polymer hydrogels for micro-patterning and tissue engineering applications
作者： Ali Fathi , Sherry Lee , Aishling Breen , Ali Negahi Shirazi , Peter Valtchev
DOI: 10.1016/J.EURPOLYMJ.2014.07.011
关键词: Osteoblast 、 Materials science 、 Self-healing hydrogels 、 Regeneration (biology) 、 Tissue engineering 、 Ethylene glycol 、 Chemical engineering 、 Polymer 、 Gelatin 、 Composite material 、 Biopolymer
摘要: Abstract Low mechanical strength and rapid degradation of photo-crosslinkable polymers are the major obstacles for their applications. The aim this study was to address these issues by fabricating hybrid polymeric hydrogels from a biopolymer (gelatin) synthetic polymer. Methacrylated gelatin (GelMA) poly(lactic-ethylene oxide fumarate) (PLEOF) were photo-crosslinked, using Irgacure poly(ethylene glycol)-diacrylate. optimum hydrogel produced when 200 mg/ml PLEOF 100 mg/ml GelMA. These possessed porosity in range 90%, also comprised micro (∼20 μm) macro pores (540 μm), which suitable nutrients mass transfer osteoblast cell proliferation, respectively. compression modulus GelMA-PLEOF more than 200 kPa, is paramount compared GelMA hydrogels. Moreover, fabrication substantially enhanced structural stability simulated physiological environment one week 28 days. In vitro studies showed that primary human cells adhered proliferated into PLEOF/GelMA hydrogel. Additionally, micro-patterns with 10 × 100 μm dimensions created on surface promote cellular alignment. results demonstrated potential construct regeneration load-bearing tissues.
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