Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos

摘要: A key challenge in the post-genomic area is to identify function of genes discovered, with many still uncharacterised all metazoans. first step transcription pattern characterisation, for which we now have near whole-genome coverage Drosophila. However, much more limited information about expression and subcellular localisation corresponding proteins. The Cambridge Protein Trap Consortium generated, via piggyBac transposition, over 600 novel YFP-trap proteins tagging just under 400 Drosophila loci. Here, characterise localisations patterns these insertions, called CPTI lines, embryos. We systematically analysed at cellularisation (stage 5) recorded stage 5, mid-embryogenesis 11) late embryogenesis (stages 15-17). At 31% nuclear lines (41) 26% cytoplasmic (67) show discrete that provide clues on protein markers organelles or regions, including nucleoli, envelope, speckles, centrosomes, mitochondria, endoplasmic reticulum, Golgi, lysosomes peroxisomes. characterised membranous/cortical (102) throughout 5 10 during epithelial morphogenesis, documenting their apico-basal position identifying those secreted extracellular space. identified tricellular vertices as a specialized membrane domain marked by integral Sidekick. Finally, categorised cytokinesis.