The Pathogenicity and Transcriptome Analysis of Methicillin-Resistant Staphylococcus aureus in Response to Water Extract of Galla chinensis

摘要: Aim. Antibiotic abuse contributes to the emergence of methicillin-resistant Staphylococcus aureus (MRSA). It is increasingly important screen new antimicrobial agents for management MRSA infections. G. chinensis, a nontoxic Chinese herbal medicine, considered potential antibacterial agent. The aim this study was investigate bactericidal effects aqueous extracts chinensis on MRSA. mechanisms extract inhibition pathogenicity in vivo are also discussed. Methods. prepared and its activities were examined by determining minimum inhibitory concentration (MIC). Biofilm biomass determined scanning electron microscopy (SEM) confocal laser (CLSM). RNA sequencing (RNA-seq) used evaluate differentially expressed functional pathways treated with extract. We validated role invasive ability using rat infectious model. Results. results indicated that sensitive at 31.25μg/mL. led reduction dextran-dependent aggregation biofilm formation Based transcriptome analysis, significantly downregulated gene expression related carbohydrate metabolism. inhibited vivo. Conclusion. properties likely modulation promising supplementary therapy lessen or eliminate use antibiotics tool