Phosphonate degradation by Spirulina strains: Cyanobacterial biofilters for the removal of anticorrosive polyphosphonates from wastewater

作者: Giuseppe Forlani , Valentina Prearo , Dorota Wieczorek , Paweł Kafarski , Jacek Lipok

DOI: 10.1016/J.ENZMICTEC.2010.12.005

关键词: Sewage treatmentBiochemistryBacteriaBiologyBioremediationMethylphosphonic acidWastewaterEnvironmental chemistryBiodegradationXenobioticBiofilter

摘要: Abstract The ability of Spirulina spp. to metabolize the recalcitrant xenobiotic Dequest 2054 ® [hexamethylenediamine-N,N,N′,N′-tetrakis(methylphosphonic acid)], a CaSO 4 inhibitor used for boiler treatment and reverse osmosis desalination, was investigated. compound served as sole source phosphorus, but not nitrogen, cyanobacterial growth. In vivo utilization followed by 31 P NMR analysis. disappearance polyphosphonate proceeded only with actively dividing cells, no release inorganic phosphate evident. However, difference found between P-starved P-fed cultures. Maximal reached 1.0 ± 0.2 mmol l −1 , corresponding 0.56 ± 0.11 mmol g dry biomass, thus residual amounts were still present in exhausted medium when supplied at higher initial concentrations. At low substrate levels metabolism rates lower, suggesting that concentration-driven uptake may represent limiting step during biodegradation process. retained biocolumns made immobilized either alive or dead. A lab-scale pilot plant, consisting series sequentially connected vessels containing an proliferating algal culture, built tested wastewater treatment. Results showed 50% removal added concentration 2.5 mM. Although further optimization will be required, data strengthen possibility using strains bioremediation purposes.

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