Chromatographic and electrophoretic studies of protein binding to chiral solutes.

摘要: Protein interactions are important in determining the transport, metabolism and/or activity of many chiral compounds within body. This review examines data that have been obtained on these by various chromatographic and electrophoretic methods, especially those based either high-performance liquid chromatography or capillary electrophoresis. Zonal elution, frontal analysis vacancy methods each considered, as approaches employ soluble immobilized proteins. There a variety different items can be learned about solute-protein system through techniques. includes information binding constants number sites for system, well thermodynamic parameters, rate constants, interaction forces site structure protein solute. Numerous examples provided throughout this review, taken from literature work performed author's laboratory.