Molecular survey of trichothecene genotypes of Fusarium graminearum species complex from barley in southern Brazil.
作者： P. Astolfi , J. dos Santos , L. Schneider , L.B. Gomes , C.N. Silva
DOI: 10.1016/J.IJFOODMICRO.2011.05.019
关键词: Strain (biology) 、 Hordeum vulgare 、 Botany 、 Genotype 、 GenBank 、 Mycotoxin 、 Veterinary medicine 、 Fusarium 、 Biology 、 Trichothecene 、 Species complex
摘要: Fusarium head blight is a disease of primary concern to small-grain cereals Brazil, including barley. Its main causal agent, graminearum species complex (Fg complex)¸ able produce mycotoxins, especially deoxynivalenol (DON) and nivalenol (NIV), that usually contaminate grain. Strains DON may also its acetylated derivatives: 3-acetyl-DON (3-ADON) 15-acetyl-DON (15-ADON). Ninety two isolates were obtained from samplings barley grain during three years (2007, 2008 2009) several fields in both southern northern production regions Rio Grande do Sul state, Brazil. These examined for polymerase chain-reaction-based (PCR) trichothecene genotype based on the amplification portions Tri3 Tri12. There was no effect year or region proportion genotypes. Overall, 66% strains (61/92) 15-ADON, 4.4% (4/92) 3-ADON 29.3% (27/92) NIV. The overall NIV/DON ratio estimated (0.41) five times higher than found previous studies with wheat grown same region. Species identification nine representing genotypes, comparisons DNA sequences PHO, RED URA genes curated reference GenBank, revealed they belong F. sensu stricto (four 15-ADON one strain), meridionale (three NIV strains) austroamericanum (one strain). results add current regional knowledge genotypes within Fg affecting
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