Heterogeneity in lymphokine profiles of CD4+ and CD8+ T cells and clones activated in vivo and in vitro.

摘要: Analysis of lymphokine mRNA expression and protein secretion by about 100 short-term alloreactive T-cell clones revealed marked heterogeneity in the combinations lymphokines synthesized. This finding argues against a simple model which T cells express either an unrestricted (Th0) or restricted (Th1 Th2) profile. Lymphokine titers appeared to be normally distributed, with percentage positive for any one product determined threshold detection. Accordingly, observation that CD4+ on average produced higher most than CD8+ indicated apparent differences between profiles these two subsets were quantitative rather qualitative. Patterns gene detected whole tissues analysis single markedly influenced vivo priming. Relative levels IL-4, IFN-gamma GM-CSF lymphoid differed mice undergoing GvHR following contact sensitization OX immunization KLH adjuvant. Consistent IL-4 was major lymph nodes KLH-primed mice, KLH-specific derived from such also expressed IL-4. A similar approach detection lymphokine-secreting precursors activated late L. infection showed major-resistant strain, C57BL/6, secreted without whereas susceptible BALB/c, IFN-gamma. Differences noted anti-CD3-induced IL-3 production at single-cell level tumor allograft. Con A-induced, filler cell-dependent cloning unprimed gave rise both IFN-gamma-producing IL-4-producing clones. requirement undefined, signal development IL-4-secreting suggested normal anti-CD3-induced, cell-free system exclusively detectable IL-6. With view developing vivo-activated cells, sensitive assays other used measure secreting allograft.(ABSTRACT TRUNCATED AT 400 WORDS)