Mutational pattern of influenza B viruses adapted to high growth replication in embryonated eggs
作者： Vladimir Y. Lugovtsev , Galina M. Vodeiko , Roland A. Levandowski
DOI: 10.1016/J.VIRUSRES.2004.11.016
关键词: Virology 、 Embryonated 、 DNA 、 Gene 、 Antigenic drift 、 Mutation 、 Sequence analysis 、 Genetics 、 Biology 、 Genotype 、 Serial passage 、 Cancer research 、 Infectious Diseases
摘要: Improved replication of influenza viruses in embryonated chicken eggs (CE) permits increased vaccine production and availability. We investigated the growth properties B relation to specific mutations occurring after serial passage CE. In experiments yielding high variants B/Victoria/504/2000, predicted alter amino acid (AA) composition occurred only near receptor-binding pocket hemagglutinins (HA) no other genes. Two B/Victoria/504/2000 had same AA substitutions HA (R162M D196Y), but higher yield variant a third substitution (G141E), which also altered antigenic characteristics. experiment B/Hong Kong/330/2001, PB2 NP domains relate RNP formation function. Our results indicate that adaptation high-yield by CE requires few either internal or external Specific modifications genes combination could be used optimize create for substrates.
elsevier.com
sci-hub.se 参考文章(62)
Paul Digard, Debra Elton, Konrad Bishop, Elizabeth Medcalf, Alan Weeds, Brian Pope, Modulation of nuclear localization of the influenza virus nucleoprotein through interaction with actin filaments. Journal of Virology. ,vol. 73, pp. 2222- 2231 ,(1999) , 10.1128/JVI.73.3.2222-2231.1999
Iain W. Mattaj, Ludwig Englmeier, Nucleocytoplasmic Transport: The Soluble Phase Annual Review of Biochemistry. ,vol. 67, pp. 265- 306 ,(1998) , 10.1146/ANNUREV.BIOCHEM.67.1.265
M T Berton, C W Naeve, R G Webster, Antigenic structure of the influenza B virus hemagglutinin: nucleotide sequence analysis of antigenic variants selected with monoclonal antibodies. Journal of Virology. ,vol. 52, pp. 919- 927 ,(1984) , 10.1128/JVI.52.3.919-927.1984
Robert E. O'Neill, Richard Jaskunas, Günter Blobel, Peter Palese, Junona Moroianu, Nuclear Import of Influenza Virus RNA Can Be Mediated by Viral Nucleoprotein and Transport Factors Required for Protein Import ( Journal of Biological Chemistry. ,vol. 270, pp. 22701- 22704 ,(1995) , 10.1074/JBC.270.39.22701
Naoko Nakagawa, Ritsuko Kubota, Toshimasa Nakagawa, Yoshinobu Okuno, Antigenic variants with amino acid deletions clarify a neutralizing epitope specific for influenza B virus Victoria group strains. Journal of General Virology. ,vol. 82, pp. 2169- 2172 ,(2001) , 10.1099/0022-1317-82-9-2169
O T Gorman, R O Donis, Y Kawaoka, R G Webster, Evolution of influenza A virus PB2 genes: implications for evolution of the ribonucleoprotein complex and origin of human influenza A virus. Journal of Virology. ,vol. 64, pp. 4893- 4902 ,(1990) , 10.1128/JVI.64.10.4893-4902.1990
Sun-Woo Yoon, Richard J. Webby, Robert G. Webster, Evolution and Ecology of Influenza A Viruses Influenza Pathogenesis and Control - Volume I. ,vol. 56, pp. 359- 375 ,(2014) , 10.1007/82_2014_396
L.J. REED, H. MUENCH, A SIMPLE METHOD OF ESTIMATING FIFTY PER CENT ENDPOINTS American Journal of Epidemiology. ,vol. 27, pp. 493- 497 ,(1938) , 10.1093/OXFORDJOURNALS.AJE.A118408
C W Mandl, E Puchhammer-Stöckl, C Kunz, F X Heinz, Sequencing the termini of capped viral RNA by 5'-3' ligation and PCR. BioTechniques. ,vol. 10, pp. 484- 486 ,(1991)
Robert A Lamb, Griffith D. Parks, Griffith D. Parks, Griffith D. Parks, Orthomyxoviridae: The Viruses and Their Replication. Fields virology. ,vol. 2, pp. 1449- 1496 ,(1996)