High-throughput analysis of the satellitome revealed enormous diversity of satellite DNAs in the neo-Y chromosome of the cricket Eneoptera surinamensis

摘要: Satellite DNAs (satDNAs) constitute large portion of eukaryote genomes, comprising non-protein-coding sequences tandemly repeated. They are mostly found in heterochromatic regions chromosomes such as around centromere or near telomeres, intercalary heterochromatin, and often non-recombining segments sex chromosomes. We examined the satellitome cricket Eneoptera surinamensis (2n = 9, neo-X1X2Y, males) to characterize molecular evolution its neo-sex To achieve this, we analyzed illumina reads using graph-based clustering complementary analyses. an unusually high number 45 families satDNAs, ranging from 4 bp 517 bp, accounting for about 14% genome showing different modular structures diversity arrays. FISH mapping revealed that satDNAs located C-positive pericentromeric SatDNAs enrichment was also observed comparison autosomes. Especially astonishing accumulation loci highly differentiated neo-Y, including 39 over-represented this chromosome, which is greatest yet reported Our results suggest possible involvement increasing differentiation species, contributing understanding chromosome composition Orthoptera.