Synthesis and hybridization of a series of biotinylated oligonucleotides.

作者: Alan F. Cook , Edmund Vuocolo , Christine L. Brakel

DOI: 10.1093/NAR/16.9.4077

关键词: BiologyNucleic acid thermodynamicsBiotinDNAPhosphoramiditeStreptavidinHorseradish peroxidaseOligonucleotideBiotinylationBiochemistryCombinatorial chemistry

摘要: … to target DNA immobilized on microtiter wells (ELISA plates), and were detected with a streptavidin-biotinylated … Control single-stranded target DNA was prepared from wild type (no lac …

参考文章(1)
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)