DNA-mediated transfer of major histocompatibility class II I-Ab and I-Abm12 genes into B lymphoma cells: molecular and functional analysis of introduced antigens.

摘要: A20.2J B lymphoma cells have been co-transfected with the A alpha b, beta b or bm12 and neomycin resistance genes. The transfected cell lines constitutively express I-Ab I-Abm12 class II molecules at a level comparable that of endogenous I-Ad antigen. antigens expressed on three independently clones (A20.Ab.1, A20.Ab.2, A20.Ab.3) are serologically functionally indistinguishable from by control H-2bxd hybridoma (LB cells). These were potent I region-restricted antigen-presenting to large panel antigen-specific, autoreactive alloreactive T hybridomas, as well normal clones. There not significant differences in efficiency antigen presentation Ia encoded transfected, compared endogenous, I-A expression functional surface genes is consistent previous work implicated beta-chain alone bm 12 mutation. Furthermore, because A20.Ab A20.Abm12 display serologic properties spleen wild-type mutant mouse strains, respectively, it clear do undergo unexpected unpredictable alterations after transfection this system. This system permits us investigate structural requirements for interactions between major histocompatibility complex antigens, foreign antigen, receptor vitro site-directed mutagenesis coupled DNA-mediated gene transfer.