Hepatitis C virus NS5A: enigmatic but still promiscuous 10 years on!

摘要: Since one of us co-authored a review on NS5A decade ago, the hepatitis C virus (HCV) field has changed dramatically, primarily due to advent JFH-1 cell culture infectious clone, which allowed study all aspects life cycle from entry exit. This will describe advances in our understanding biology over past decade, highlighting how system determine that is essential not only genome replication but also assembly virions. We shall recent structural insights - predicted comprise three domains; X-ray crystallography revealed structure domain I there lack detailed information about other two domains, are be largely unstructured. Recent into phosphorylation discussed, and we highlight few pertinent examples ever-expanding list NS5A-binding partners identified decade. Lastly, literature showing potential target for new class highly potent small molecules function inhibit replication. These direct-acting antivirals (DAAs) now either licensed, or late stages approval clinical use both USA UK/Europe. In combination with DAAs targeting viral protease (NS3) polymerase (NS5B), they revolutionizing treatment HCV infection.