Upregulation of Vascular Angiotensin II Receptor Gene Expression by Low-Density Lipoprotein in Vascular Smooth Muscle Cells

摘要: Background Because LDL and the angiotensin II type 1 (AT1) receptor are thought to be involved in pathogenesis of chronic vascular disease, we studied possible interactions between these two biological systems cultured rat smooth muscle cells. Methods Results Incubation cells with 100 μg/mL profoundly increased AT1 mRNA ≈250% control levels as assessed by Northern hybridization analysis. This effect is maximal 12 hours after addition culture medium sustained for up 24 hours. The LDL-induced upregulation dose dependent, a obtained LDL. There correlative increase cell surface–associated receptors saturation radioligand binding assays. half-life substantially compared that treated only 5,6-dichlorobenzimidazole block transcription. Angiotensin II–induced elevation cytosolic calcium concen...