Fluorometric assay of sialic acid in the picomole range: A modification of the thiobarbituric acid assay
作者： K.S. Hammond , D.S. Papermaster
DOI: 10.1016/0003-2697(76)90210-4
关键词: Fluorescence 、 Sialic acid 、 Reaction product 、 Chromatography 、 Mass spectrometry 、 Cellular material 、 Chemistry 、 Thiobarbituric acid
摘要: Abstract Fluorescence of the thiobarbituric acid reaction product periodate-cleaved sialic is readily detected by excitation at 550 nm and analysis emitted light 570 nm. A fluorometric microassay described which 500-fold more sensitive than usual spectrophotometric procedures detects 10 ng (32 pmol) acid. Contamination 2-deoxyribose from cellular material easily shifts maxima below
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