Isolation and characterization of a satellite DNA family in the Saccharum complex
作者： Karine Alix , Franc-Christophe Baurens , Florence Paulet , Jean-Christophe Glaszmann , Angélique D'Hont
DOI: 10.1139/GEN-41-6-854
关键词: Satellite DNA 、 Saccharum 、 genomic DNA 、 Southern blot 、 Sequence alignment 、 Genetics 、 Helictotrichon 、 Saccharum spontaneum 、 Biology 、 Saccharum officinarum
摘要: EaCIR1, a 371-bp Erianthus-specific satellite DNA sequence, was cloned from TaqI restricted genomic after agarose-gel electrophoresis. This sequence has 77% homology with 365-bp of Helictotrichon convolutum and 72% 353-bp tandem repeat Oryza sativa. PCR primers defined in the conserved regions these repetitive sequences were used to isolate other DNAs different representatives Saccharum complex: SoCIR1 officinarum, SrCIR1 robustum, SsCIR1 SsCIR2 spontaneum, MsCIR1 Miscanthus sinensis. EaCIR1 localized subtelomeric chromosomes by fluorescence situ hybridization. Southern hybridization experiments, using two this family as probes, illustrated contrasting species-specificity demonstrated existence similar elements sorghum maize.
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