Endothelin-induced changes of second messengers in cultured human ciliary muscle cells.
作者： T Yorio , L DeSantis , I H Pang , S Matsumoto , P E Magnino
DOI:
关键词: Adenylyl cyclase 、 Prostaglandin E 、 Inositol 、 Internal medicine 、 Biology 、 Endothelin receptor 、 Calcium in biology 、 Second messenger system 、 Cyclic adenosine monophosphate 、 Phospholipase C 、 Endocrinology
摘要: Purpose. To characterize the pharmacology of endothelin-induced changes in phospholipase C (PLC) activity, intracellular calcium concentration ([Ca 2+ ] i ), and cyclic adenosine monophosphate (cAMP) cultured human ciliary muscle (HCM) cells. Methods. Changes PLC activity HCM cells were determined by production [ 3 H]inositol phosphates. [Ca was single-cell dynamic fluorescence ratio imaging. Radioimmunoassays used to determine cAMP prostaglandin E 2 (PGE ) concentrations. Results. Endothelin-1 (ET-1) stimulated (mean EC 50 = 335 pM) activated mobilization These effects mediated endothelin ET A receptor subtype because an B receptor-selective agonist, sarafotoxin S6c, ineffective. Additionally, ET-1 inhibited pretreatment with a selective antagonist, BQ610 pK 9.96 for PLC). also PGE 12.0 nM) 5.2 these appeared mediate stimulatory effect on adenylyl cyclase blockade ET-1-induced 10 μM indomethacin completely blocked ET-1-activated production. Conclusions. increased subtype. production, likely enhanced prostaglandins.
arvojournals.org参考文章(30)
J S Schuman, K Erickson-Lamy, C Korbmacher, J A Nathanson, Effect of endothelin on outflow facility and accommodation in the monkey eye in vivo. Investigative Ophthalmology & Visual Science. ,vol. 32, pp. 492- 495 ,(1991)
Y. Kitazawa, T. Taniguchi, Mohammad Sabbir Reza Haque, K. Sugiyama, K. Okada, The ocular hypotensive effect of the ETB receptor selective agonist, sarafotoxin S6c, in rabbits. Investigative Ophthalmology & Visual Science. ,vol. 36, pp. 804- 808 ,(1995) , 10.11501/3133631
Ernst R. Tamm, L. DeSantis, I. H. Pang, D. L. Shade, Single-cell contraction assay for human ciliary muscle cells : effect of carbachol Investigative Ophthalmology & Visual Science. ,vol. 34, pp. 1876- 1879 ,(1993)
E H Bárány, P L Kaufman, Loss of acute pilocarpine effect on outflow facility following surgical disinsertion and retrodisplacement of the ciliary muscle from the scleral spur in the cynomolgus monkey. Investigative Ophthalmology & Visual Science. ,vol. 15, pp. 793- 807 ,(1976)
T. Yorio, L. Desantis, Iok-Hou Pang, S. Matsumoto, Muscarinic Effects on Cellular Functions in Cultured Human Ciliary Muscle Cells Investigative Ophthalmology & Visual Science. ,vol. 35, pp. 3732- 3738 ,(1994)
U Chakravarthy, A J Douglas, J R Bailie, D B Archer, B McKibben, Immunoreactive endothelin distribution in ocular tissues. Investigative Ophthalmology & Visual Science. ,vol. 35, pp. 2448- 2454 ,(1994)
S. Karne, C.K. Jayawickreme, M.R. Lerner, Cloning and characterization of an endothelin-3 specific receptor (ETC receptor) from Xenopus laevis dermal melanophores. Journal of Biological Chemistry. ,vol. 268, pp. 19126- 19133 ,(1993) , 10.1016/S0021-9258(17)46743-3
Hiromi Kohmoto, Shun Matsumoto, Takashi Serizawa, Effects of endothelin-1 on [Ca2+]i and pHi in trabecular meshwork cells. Current Eye Research. ,vol. 13, pp. 197- 202 ,(1994) , 10.3109/02713689408995777
Marcello D. Lograno, Alfredo Reibaldi, Receptor-responses in fresh human ciliary muscle. British Journal of Pharmacology. ,vol. 87, pp. 379- 385 ,(1986) , 10.1111/J.1476-5381.1986.TB10827.X
Frank Stahl, Barbara Gebauer, Albrecht Lepple-Wienhues, Gunhild Langenbeck-Groh, Susanne Berweck, Michael Wiederholt, Characterization of acetylcholine- and endothelin-induced calcium entry in cultured human ciliary muscle cells. Pflügers Archiv: European Journal of Physiology. ,vol. 422, pp. 105- 111 ,(1992) , 10.1007/BF00370409