DNA stainability with base-specific fluorochromes: dependence on the DNA topology in situ.
作者： E. Prosperi , M. C. Giangar� , G. Bottiroli
DOI: 10.1007/BF00269016
关键词: Binding site 、 Förster resonance energy transfer 、 HeLa 、 Chromatin 、 Molecular biology 、 Chemistry 、 Plasmid 、 Propidium iodide 、 PBR322 、 Topology 、 DNA
摘要: The influence of DNA topology on stainability with the externally binding fluorochromes Hoechst 33258 (HO) and mithramycin (MI) was investigated in HeLa nuclei comparison intercalating dye propidium iodide (PI). Changes were induced a mild DNAse I treatment. Stainability properties untreated nuclease-treated compared those supercoiled-circular relaxed-linear forms plasmid pBR322. DNAse-treated stained HO showed higher fluorescence intensity than control samples, independently concentration, contrast findings obtained PI. Similar behaviour observed form pBR322, molecule. With MI, did not depend topology, whereas similar to that HO. In order assess whether this discrepancy depended differences availability DNAse-sensitive sites fluorochromes, resonance energy transfer (FRET) studies performed HO+PI, or HO+MI pairs. After digestion, relative FRET efficiency between donor acceptor molecules (PI MI) reduced significantly only when MI acceptor. This result may be due greater MI. These indicate base-specific affected by chromatin regions.
springer.com
sci-hub.se 参考文章(43)
J.G. Wang, The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients. Journal of Molecular Biology. ,vol. 89, pp. 783- 801 ,(1974) , 10.1016/0022-2836(74)90053-9
Jean-Bernard Le Pecq, Ethidium Bromide: A Fluorescent Probe of Nucleic Acid Structure and Its Potential for in-Vivo Studies Springer, Berlin, Heidelberg. pp. 301- 309 ,(1973) , 10.1007/978-3-642-49204-4_30
William Bauer, Jerome Vinograd, The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye Journal of Molecular Biology. ,vol. 33, pp. 141- 171 ,(1970) , 10.1016/0022-2836(68)90286-6
D.A. Jackson, P. Dickinson, P.R. Cook, The size of chromatin loops in HeLa cells The EMBO Journal. ,vol. 9, pp. 567- 571 ,(1990) , 10.1002/J.1460-2075.1990.TB08144.X
I.A. Brazell, P.R. Cook, E. Jost, Characterization of nuclear structures containing superhelical DNA. Journal of Cell Science. ,vol. 22, pp. 303- 324 ,(1976) , 10.1242/JCS.22.2.303
B Villeponteau, H G Martinson, Gamma rays and bleomycin nick DNA and reverse the DNase I sensitivity of beta-globin gene chromatin in vivo. Molecular and Cellular Biology. ,vol. 7, pp. 1917- 1924 ,(1987) , 10.1128/MCB.7.5.1917
Trond Stokke, Harald B. Steen, Binding of Hoechst 33258 to chromatin in situ Cytometry. ,vol. 7, pp. 227- 234 ,(1986) , 10.1002/CYTO.990070302
Michael W. Van Dyke, Peter B. Dervan, Chromomycin, mithramycin, and olivomycin binding sites on heterogeneous DNA. Footprinting with methidiumpropyl-EDTA.cntdot.iron(II) Biochemistry. ,vol. 22, pp. 2373- 2377 ,(1983) , 10.1021/BI00279A011
Xiaolian Gao, Dinshaw J. Patel, Solution structure of the chromomycin-DNA complex. Biochemistry. ,vol. 28, pp. 751- 762 ,(1989) , 10.1021/BI00428A051
Cheeptip Benyajati, Abraham Worcel, Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster Cell. ,vol. 9, pp. 393- 407 ,(1976) , 10.1016/0092-8674(76)90084-2