Lysis of Escherichia coli by Ethylenediaminetetraacetate and Phospholipases as Measured by β-Galactosidase Activity

摘要: A permeaseless mutant of Escherichia coli, which produces β-galactosidase constitutively, was treated briefly with ethylenediaminetetraacetate and then the phospholipases Bacillus cereus. Cell lysis occurred, as indicated by an increase in activity a decrease absorbancy cell suspension. The susceptibility cells to attack markedly affected age when harvested. results suggest that permeability changes may be associated phospholipase specifically degrades phosphatidyl ethanolamine. sonic-treatment method for determining total content E. coli cells, is independent their harvested, described.