Reverse genetics studies on the filamentous morphology of influenza A virus.

摘要: We have investigated the genetic determinants responsible for filamentous morphology of influenza A viruses, a property characteristic primary virus isolates. plasmid-based reverse genetics system was used to transfer M segment A/Udorn/72 (H3N2) into A/WSN/33 (H1N1) virus. While WSN virions display spherical morphology, recombinant WSN-Mud acquired ability parental Udorn strain form particles. This determined by immunofluorescence studies in infected MDCK cells and electron microscopy purified To determine gene product within we generated four viruses carrying different sets M1 M2 genes from or strains background. These revealed that Udorn, independently origin gene, conferred budding properties viruses. also constructed two encoding chimeric proteins containing amino-terminal 1–162 amino acids carboxy-terminal 163–252 amino protein. Neither these phenotypes, indicating both amino- domains protein contribute morphology. next rescued seven mutant WSN-M1ud single amino acid substitutions corresponding differences with Characterization residues 95 204 are critical determining particle formation.