Characteristic proteins of micronemes and dense granules from Sarcocystis tenella zoites (Protozoa, Coccidia).
作者： Jean-Francois Dubremetz , Claude Dissous
DOI: 10.1016/0166-6851(80)90061-4
关键词: Cell fractionation 、 Protein content 、 Coccidia 、 Biology 、 Microbiology 、 Electrophoresis 、 Sarcocystis tenella 、 Protozoa
摘要: Abstract A subcellular fractionation procedure has been developed, which allowed the recovery of purified fractions micronemes, dense granules and pellicles from Sarcocystis tenella zoites. As expected, sodium dodecyl sulphate polyacrylamide gels electrophoresis showed a fairly heterogenous protein content. In contrast, only one major component (42 000 daltons) was found in granules, whereas two bands (20 22 were observed for micronemes. These characteristic proteins also components whole zoite might have important functions physiology organism.
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