Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay
作者： Alexandre Melnikov , Anand Murugan , Xiaolan Zhang , Tiberiu Tesileanu , Li Wang
DOI: 10.1038/NBT.2137
关键词: Enhancer 、 Reporter gene 、 Massively parallel 、 Dissection (medical) 、 Transcription factor 、 Genetics 、 Biology 、 Computational biology 、 Genome 、 Biotechnology 、 Molecular medicine 、 Applied Microbiology and Biotechnology 、 Bioengineering 、 Biomedical engineering
摘要: An improved understanding of enhancers in mammalian genomes could facilitate the design new regulatory elements. Melnikov et al. synthesize thousands ~90 nt enhancer variants, assay their activity human cells and use data to rationally optimize synthetic enhancers.
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