Multi-platform metabolomics assays for human lung lavage fluids in an air pollution exposure study

摘要: Metabolomics protocols are used to comprehensively characterize the metabolite content of biological samples by exploiting cutting-edge analytical platforms, such as gas chromatography (GC) or liquid (LC) coupled mass spectrometry (MS) assays, well nuclear magnetic resonance (NMR) assays. We have developed novel sample preparation procedures combined with GC-MS, LC-MS, and NMR metabolomics profiling for analyzing bronchial wash (BW) bronchoalveolar lavage (BAL) fluid from 15 healthy volunteers following exposure biodiesel exhaust filtered air. Our aim was investigate responsiveness profiles in human lung air pollution derived combustion biofuels, rapeseed methyl ester biodiesel, which increasingly being promoted alternatives conventional fossil fuels. multi-platform approach enabled us detect greatest number unique metabolites yet reported BW BAL (82 total). All assays indicated that fluids differed appreciably, 46 showing significantly different levels corresponding compartments. Furthermore, GC-MS assay revealed an effect on 1-monostearylglycerol, sucrose, inosine, nonanoic acid, ethanolamine (in BAL) pentadecanoic acid BW), whereas LC-MS a shift niacinamide BAL). The only identified lactic BW) responsive exposure. findings demonstrate proposed is useful wide screening can facilitate elucidation Graphical Abstract abstract illustrating study workflow. Nuclear Magnetic Resonance, LC-TOFMS Liquid chromatography-Time Of Flight Mass Spectrometry, GC Gas Chromatography-Mass spectrometry.