Successful orthotopic uterine allotransplantation in a rabbit model using aorta and cava anastomoses: a short-term viability study.

摘要: Purposes We aim to develop a rabbit model system for studies on uterus transplantation. Methods Six sexually mature female New Zealand white rabbits of proven fertility were used harvesting the uterine allograft with an aortic-caval macrovascular patch (including aorta, inferior vena cava, common and internal iliacs, arterial venous tree). The patches transplanted in six recipients orthotopically using aorta cava anastomoses. Tacrolimus (0.5 mg twice daily, p.o.) was administered postoperatively immunosuppression. Results Surgical survival 100% (n = 6), graft rate 83.3% 5). No died intraoperatively, but only one achieved short-term (for 8 days). Four (#1, #2, #3 #4) within first 24 h as result veno-vena anastomosis breakdown, bradycardia, intestinal necrosis, respiratory failure, respectively. Rabbits #5 (30 h) #6 (8 days) from obstruction pneumonia, Uterine morphology normal #6, rejection not observed grafted uterus, which further verified by H&E immunohistochemistry. Conclusions Aorta anastomoses can be ensure viable reconstructing adequate blood supply model. have demonstrated feasibility tacrolimus monotherapy suppressing allotransplanted However, UTx seems difficult achieve long-term survival.