Chemiluminescence flow biosensor for determination of total d-amino acid in serum with immobilized reagents
作者： Baoxin Li , Zhujun Zhang
DOI: 10.1016/S0925-4005(00)00405-6
关键词: Silica gel 、 Hydrogen peroxide 、 Chemiluminescence 、 Luminol 、 Amino acid 、 Ferricyanide 、 Detection limit 、 Chromatography 、 Alanine 、 Chemistry
摘要: A flow-injection chemiluminescence (CL) biosensor for the determination of total d-amino acid is described in this paper. d-amino acid oxidase was immobilized onto amine-modified silica gel via glutaraldehyde activation and packed in a column. The other analytical reagents, including luminol and ferricyanide, were electrostatically co-immobilized on an anion-exchange column. d-amino acid was sensed by the CL reaction between hydrogen peroxide produced from the enzyme column and luminol and ferricyanide, which were …
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