Electrospun SF/PLCL nanofibrous membrane: a potential scaffold for retinal progenitor cell proliferation and differentiation

摘要: Biocompatible polymer scaffolds are promising as potential carriers for the delivery of retinal progenitor cells (RPCs) in cell replacement therapy repair damaged or diseased retinas. The primary goal present study was to investigate effects blended electrospun nanofibrous membranes silk fibroin (SF) and poly(L-lactic acid-co-e-caprolactone) (PLCL), a novel scaffold, on biological behaviour RPCs vitro. To assess cell-scaffold interaction, were cultured SF/PLCL indicated durations. Our data revealed that all thoroughly cytocompatible, SF:PLCL (1:1) yielded best RPC growth. vitro proliferation assays showed proliferated more quickly than other control. Quantitative polymerase chain reaction (qPCR) immunocytochemistry analyses demonstrated grown preferentially differentiated toward neurons, including, most interestingly, photoreceptors. In summary, we can not only markedly promote with cytocompatibility growth but also robustly enhance RPCs' differentiation specific neurons interest vitro, suggesting may have applications future.