A multiplex PCR assay for simultaneous detection of Escherichia coli O157:H7, Bacillus cereus, Vibrio parahaemolyticus, Salmonella spp., Listeria monocytogenes, and Staphylococcus aureus in Korean ready-to-eat food.

作者: Nari Lee , Kyung Yoon Kwon , Su Kyung Oh , Hyun-Joo Chang , Hyang Sook Chun

DOI: 10.1089/FPD.2013.1638

关键词: HemolysinEscherichia coliBacillus cereusBiologySalmonellaMicrobiologyMolecular biologyListeria monocytogenesMultiplex polymerase chain reactionMultiplexVibrio parahaemolyticus

摘要: … The primer pairs selected in this study were rfb-F/R for amplification of a 678-bp sequence from the rfbE gene of E. coli O157:H7, gyr-F/R for amplification of a 540-bp sequence from the …

参考文章(26)
Isao Nagano, Miyuki Kunishima, Yoshihiro Itoh, Zhiliang Wu, Yuzo Takahashi, Detection of Verotoxin-Producing Escherichia coli O157:H7 by Multiplex Polymerase Chain Reaction Microbiology and Immunology. ,vol. 42, pp. 371- 376 ,(1998) , 10.1111/J.1348-0421.1998.TB02297.X
Susumu Kawasaki, Pina M. Fratamico, Naoko Horikoshi, Yukio Okada, Kazuko Takeshita, Takashi Sameshima, Shinichi Kawamoto, Evaluation of a multiplex PCR system for simultaneous detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in foods and in food subjected to freezing. Foodborne Pathogens and Disease. ,vol. 6, pp. 81- 89 ,(2009) , 10.1089/FPD.2008.0153
Hiroshi Kobayashi, Jun Kubota, Kumiko Fujihara, Ken-ichi Honjoh, Masayoshi Iio, Naomi Fujiki, Mika Nakabe, Shun-ichi Oda, Toshiya Satoyama, Kazushige Takasu, Hisao Nakanishi, Takahisa Miyamoto, Simultaneous Enrichment of Salmonella spp, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes by Single Broth and Screening of the Pathogens by Multiplex Real-time PCR Food Science and Technology Research. ,vol. 15, pp. 427- 438 ,(2009) , 10.3136/FSTR.15.427
Yigang Xu, Lichun Cui, Changyong Tian, Sulong Li, Jijuan Cao, Zhongmei Liu, Guocai Zhang, A multiplex polymerase chain reaction coupled with high-performance liquid chromatography assay for simultaneous detection of six foodborne pathogens Food Control. ,vol. 25, pp. 778- 783 ,(2012) , 10.1016/J.FOODCONT.2011.12.014
Andrea Germini, Annalisa Masola, Paola Carnevali, Rosangela Marchelli, Simultaneous detection of Escherichia coli O175:H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR Food Control. ,vol. 20, pp. 733- 738 ,(2009) , 10.1016/J.FOODCONT.2008.09.010
VK Sharma, EA Dean-Nystrom, TA Casey, Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other shiga toxigenic E. coli. Molecular and Cellular Probes. ,vol. 13, pp. 291- 302 ,(1999) , 10.1006/MCPR.1999.0251
J. McLauchlin, The relationship between Listeria and listeriosis Food Control. ,vol. 7, pp. 187- 193 ,(1996) , 10.1016/S0956-7135(96)00038-2