DEVELOPMENTAL POTENTIAL OF CUMULUS CELL-DERIVED CULTURED CELLS FROZEN IN A QUIESCENT STATE AFTER NUCLEUS TRANSFER
作者： T. Tani , Y. Kato , Y. Tsunoda
DOI: 10.1016/S0093-691X(00)00302-2
关键词: Embryo 、 Cytoplast 、 Biology 、 Somatic cell 、 Mitosis 、 Molecular biology 、 Nucleus 、 Cryopreservation 、 Andrology 、 Blastocyst 、 In vitro
摘要: An efficient method for freezing donor cells is necessary when using nucleus transfer of somatic large-scale cloning. In the present study, we developed a and thawing bovine cumulus cell-derived cultured to be used as donors. Cumulus were obtained from ovaries living slaughtered in vitro. serum-starved several days induce quiescent state then frozen at -70 degrees C least 2 d. Immediately thereafter or h after thawing, nuclear without additional vitro culture. The fusion rate with recipient cytoplasts was not affected by cell source (slaughtered living) time (0 h). cleavage frozen-thawed cows immediately h) highest (97%) significantly higher than that controls (85%) transferred (85%). There no significant differences among any groups potential embryos develop into blastocysts (34 vs 44 44%, 39 45 46%). Thus, storage effective might useful convenient maximum periods developmental such transfers requires further examination.
cabdirect.org
elsevier.com
sci-hub.se 参考文章(11)
T. Wakayama, A. C. F. Perry, M. Zuccotti, K. R. Johnson, R. Yanagimachi, Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei Nature. ,vol. 394, pp. 369- 374 ,(1998) , 10.1038/28615
C.F. Rosenkrans, N.L. First, Culture of bovine zygotes to the blastocyst stage: Effects of amino acids and vitamins☆ Theriogenology. ,vol. 35, pp. 266- ,(1991) , 10.1016/0093-691X(91)90242-6
A.K. Tarkowski, An Air-Drying Method for Chromosome Preparations from Mouse Eggs Cytogenetic and Genome Research. ,vol. 5, pp. 394- 400 ,(1966) , 10.1159/000129914
K. H. S. Campbell, J. McWhir, W. A. Ritchie, I. Wilmut, Sheep cloned by nuclear transfer from a cultured cell line Nature. ,vol. 380, pp. 64- 66 ,(1996) , 10.1038/380064A0
I. Wilmut, A. E. Schnieke, J. McWhir, A. J. Kind, K. H. S. Campbell, Viable offspring derived from fetal and adult mammalian cells Nature. ,vol. 385, pp. 810- 813 ,(1997) , 10.1038/385810A0
Hanna Bałakier, Maria Zenzes, Peng Wang, Neil J. MacLusky, Robert F. Casper, The effect of cryopreservation on the development of S- and G2-phase mouse embryos. Journal of Assisted Reproduction and Genetics. ,vol. 8, pp. 89- 95 ,(1991) , 10.1007/BF01138661
Yoko Kato, Tetsuya Tani, Yusuke Sotomaru, Kazuo Kurokawa, Jun-ya Kato, Hiroshi Doguchi, Hiroshi Yasue, Yukio Tsunoda, Eight Calves Cloned from Somatic Cells of a Single Adult Science. ,vol. 282, pp. 2095- 2098 ,(1998) , 10.1126/SCIENCE.282.5396.2095
B.A. Wolfe, D.C. Kraemer, Methods in bovine nuclear transfer Theriogenology. ,vol. 37, pp. 5- 15 ,(1992) , 10.1016/0093-691X(92)90243-K
David N. Wells, Pavla M. Misica, H. Robin Tervit, Production of Cloned Calves Following Nuclear Transfer with Cultured Adult Mural Granulosa Cells Biology of Reproduction. ,vol. 60, pp. 996- 1005 ,(1999) , 10.1095/BIOLREPROD60.4.996
W I Kuczyński, J Ratajczak, M Z Ratajczak, D A Kregenow, A M Gewirtz, The storage of cells from different tumor lines in a mechanical freezer at -80 degrees C. Comparison to cryopreservation in liquid nitrogen. Polish journal of medicine and pharmacy. ,vol. 26, pp. 69- 72 ,(1994)