The effects of an advanced uterine environment on embryonic survival in the mare

作者: S. WILSHER , A.-C. LEFRANC , W. R. ALLEN

DOI: 10.1111/J.2042-3306.2011.00473.X

关键词: UterusPregnancyGynecologyBiologyEmbryo transferEmbryoAndrologyEmbryogenesisGestationConceptusTransplantation

摘要: Summary Reasons for performing the study: During embryo transfer (ET) equine can tolerate a wide degree of negative asynchrony but positive >2 days usually results in embryonic death. There is still confusion over whether this due to inability induce luteostasis or an inappropriate uterine environment. Objectives: To assess survival and development advanced environment. Hypothesis: Embryo–uterine asynchrony, not embryo's luteostasis, responsible death recipient mares with chronologically uterus. Methods: Experiment 1: Thirteen Day 7 embryos were transferred uteri luteal prolongation, occasioned by manual crushing their own conceptus, such that donor–recipient was between +13 +49 days. 2: carrying conceptus at Days 18 (n = 2), 15 14 4), 12 4) 11 gestation. In addition, 8 4 pregnant on gestation. Results: No pregnancies resulted following recipients prolonged dioestrus asynchronies However, use early as 5/20 (25%) twin pregnancies, which came from recipient. All showed retarded growth, occurring 4/5 (80%). Conclusions and potential relevance: The emphasise importance appropriate environment growth survive uterus even when achieved. It possible normal, non-ET pregnancy, embryo–uterine may account some cases

参考文章(65)
T. A. E. STOUT, Equine embryo transfer: review of developing potential Equine Veterinary Journal. ,vol. 38, pp. 467- 478 ,(2010) , 10.2746/042516406778400529
A-C. Lefranc, W. R. Allen, Nonpharmacological suppression of oestrus in the mare. Equine Veterinary Journal. ,vol. 36, pp. 183- 185 ,(2010) , 10.2746/0425164044868675
Sandra Wilsher, Amber Clutton-Brock, W R Allen, Successful transfer of day 10 horse embryos: influence of donor–recipient asynchrony on embryo development Reproduction. ,vol. 139, pp. 575- 585 ,(2010) , 10.1530/REP-09-0306
L.A. Silva, E.L. Gastal, M.A. Beg, O.J. Ginther, Changes in Vascular Perfusion of the Endometrium in Association with Changes in Location of the Embryonic Vesicle in Mares Biology of Reproduction. ,vol. 72, pp. 755- 761 ,(2005) , 10.1095/BIOLREPROD.104.036384
Palmer E, Chevalier F, Ultrasonic echography in the mare. Journal of reproduction and fertility. ,vol. 32, pp. 423- 430 ,(1982)
Hall Wh, Bovine superfetation by natural conception secondary to an embryo transfer pregnancy. The Cornell veterinarian. ,vol. 77, pp. 282- 283 ,(1987)
Heap Rb, Hamon M, Allen Wr, Studies on oestrogen synthesis by the preimplantation equine conceptus. Journal of reproduction and fertility. ,vol. 32, pp. 343- 352 ,(1982)
Squires El, McKinnon Ao, Morphologic assessment of the equine embryo Javma-journal of The American Veterinary Medical Association. ,vol. 192, pp. 401- ,(1988)
Flood Pf, Irvine Ds, Betteridge Kj, Oestrogens and androgens in blastocoelic fluid and cultures of cells from equine conceptuses of 10-22 days gestation. Journal of reproduction and fertility. pp. 413- ,(1979)