The steroid metabolome of pregnancy, insights into the maintenance of pregnancy and evolution of reproductive traits.

摘要: Abstract Modes of mammalian reproduction are diverse and not always conserved among related species. Progesterone is universally required to supports pregnancy but sites synthesis metabolic pathways vary widely. The steroid metabolome mid- late gestation was characterized, focusing on 5α-reduced pregnanes in species representing the Perissodactyla, Cetartiodactyla Carnivora using mass spectrometry. Metabolomes steroidogenic enzyme orthologue sequences were used heirarchial analyses. Steroid metabolite profiles similar within orders, whales cetartiodactyls for instance, with notable exceptions such as rhinoceros clustering goats, tapirs pigs. Steroidogenic sequence reflected expected evolutionary relationships once again exceptions. Human (expected outgroups) clustered perissodactyl CYP11A1, CYP17A1 SRD5A1 gene orthologues, forming outgroups only HSD17B1 SRD5A2. Spotted hyena CYP19A1 between equid whereas Carnivora. This variability highlights random adoption divergent physiological strategies evolved genetically