RELATION OF STRUCTURE TO FUNCTION IN BACTERIAL O ANTIGENS I. Isolation Methods

摘要: Abstract Nowotny, Anna M. (City of Hope Medical Center, Duarte, Calif.), Scott Thomas, Olga S. Duron, and Alois Nowotny. Relation structure to function in bacterial O antigens. I. Isolation methods. J. Bacteriol. 85:418–426. 1963.—Six isolation methods, four previously described two reported herein, were compared for the extraction endotoxic antigens from three different enterobacteria: Serratia marcescens Bizio, Salmonella typhosa O901, Escherichia coli K-12 lambda. Large variations observed toxin yield, serologically reactive material nucleic acid-releasing abilities six methods when applied strains. Chemical analyses toxic included quantitative determination amino acids, long- short-chain carboxylic carbohydrates. Comparisons acid content also made. Although chemical composition preparations was quite different, no correlation could be found between percentage above components lethal toxicity or serological reactivity. It concluded that structural elements properties responsible these biological activities cannot detected by gross analyses.