Untersuchungen zum Mechanismus der L-Agglutination

摘要: Abstract The mechanism of L-agglutination (LA) was studied by fractionating human sera means Sephadex G 200 gel filtration. LA found to be mediated 2 factors: the rheumatoid factor (RF) as a macroglobulin, occuring only in from patients with arthritis, and an IgG globulin, also normal sera, probably higly prevalent incomplete streptococcal antibody. RF-containing IgM fractions, themselves devoid activity, can rendered positive not same serum, but or commercial IgG. After absorption containing RF streptococci disappears; addition serum titers were attained similar those respective native serum. A treatment mercaptoethanol leads largely parallel disappearance latex fixation. Three substrains Streptococcus pyogenes SF 130 (type 1), varying their endowment type-specific antigens, reacted similarly LA.