Rapid Analysis of the DNA Binding Specificities of Transcription Factors with DNA Microarrays

摘要: We developed a new DNA microarray-based technology, called protein binding microarrays (PBMs), that allows rapid, high-throughput characterization of the in vitro binding-site sequence specificities transcription factors single day. Using PBMs, we identified yeast Abf1, Rap1 and Mig1. Comparison these proteins' sites with their vivo indicates PBM-derived can accurately reflect specificities. In addition to previously targets, Mig1 bound 107, 90 75 putative target intergenic regions, respectively, many which were upstream uncharacterized open reading frames. Comparative analysis indicated newly are highly conserved across five sequenced sensu stricto species and, therefore, probably functional may be used condition-specific manner. Similar PBM experiments should useful identifying cis regulatory elements transcriptional networks various genomes.