Polymerase Chain Reaction-Free, Sample-to-Answer Bacterial Detection in 30 Minutes with Integrated Cell Lysis

摘要: An important goal for improved diagnosis and management of infectious disease is the development rapid accurate technologies decentralized detection bacterial pathogens. Most current clinical methods that identify strains require time-consuming culture sample or procedures involving polymerase chain reaction.(1-3) Neither these approaches has enabled testing at point-of-need because requirement skilled technicians laboratory facilities. Here, we demonstrate performance an effective, integrated platform bacteria combines a universal lysis approach sensitive nanostructured electrochemical biosensor. The rapid, effective releasing intercellular RNA from samples, can be performed in simple, cost-effective device with analysis chip. was directly challenged unpurified lysates buffer urine. We successfully detected pres...